Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI
نویسندگان
چکیده
منابع مشابه
Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration.
BACKGROUND A number of factors influence the success of oocyte cryopreservation and subsequent ICSI. The aim of the present study is to establish the ideal time, after oocyte retrieval, for human oocyte cryopreservation via slow freezing/rapid thawing protocol with 0.3 M sucrose concentration in cryoprotectant solution (SF/RT 0.3 M). METHODS Retrospective study with 75 patients on the clinica...
متن کاملClinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration.
BACKGROUND Recently, interest in oocyte cryopreservation has steadily increased. Newly developed protocols have dramatically improved survival rates, removing perhaps the major hurdle that has prevented this approach from becoming a fully established form of treatment. However, the clinical efficiency of these protocols has not been exhaustively explored and therefore remains controversial. M...
متن کاملHuman oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method.
A slow freezing/rapid thawing method for the cryopreservation of human oocytes has been employed using a sodium-depleted culture media. In 53 frozen egg-embryo transfer (FEET) cycles, a 60.4% survival rate post-thaw was obtained and a 62.0% fertilization rate following intracytoplasmic sperm injection. Overall pregnancy rates were 26.4% per thaw attempt, 30.4% per patient, and 32.6% per embryo ...
متن کاملProspective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.
OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...
متن کاملCryopreservation of human failed-matured oocytes followed by in vitro maturation: vitrification is superior to the slow freezing method
BACKGROUND Oocyte cryopreservation is an important method used in a number of human fertility circumstances. Here, we compared the survival, in vitro maturation, fertilization, and early embryonic development rates of frozen-thawed human immature oocytes using two different cryopreservation methods. METHODS A total of 454 failed-matured oocytes [germinal vesicle (GV) and metaphase I (MI) stag...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2005
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/deh884